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Can MethPrimer be used for plant?


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#1 Xiaobao Ying

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Posted 06 December 2004 - 07:10 AM

Hello, I want to disign primers for methylation analyse of PDS, a gene of aribidopsis. I don't know whether the program MethPrimer is suitable. The methylation of C in plants is in the sequences of CNGs, CGs and CNNs. Thanks for your time and consideration

Edited by Xiaobao Ying, 06 December 2004 - 07:41 AM.


#2 pcrman

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Posted 06 December 2004 - 09:03 AM

MethPrimer and other similar primer design programs may not be well suited for plant DNA because only CpG methylation is considered in those programs. If you couldn't find a program, just pick your primers manually by following basic rules for primer design.

#3 Xiaobao Ying

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Posted 06 December 2004 - 06:52 PM

Thanks for your suggestion. Could you tell me the basic rules for primer design?




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