Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

DNA repeat screening in GC-rich promoterregion

  • Please log in to reply
No replies to this topic

#1 Henkjan



  • Members
  • Pip
  • 1 posts

Posted 06 December 2004 - 12:05 AM

I found a 9bp repeat (3-7x) in a promoterregion. The repeat itself is GC-rich as is the surrounding sequence. Because of the GC-content I had to design primers which yield a product of 420bp, a bit too long for a 9 bp repeat. Nested PCR's yielded additional interfering PCR products (I tried different annealing temp). I have tried several primersets to amplify the repeat but I can't get rid of the heteroduplexes also. I have tried different urea gels with urea and/or formamide loading buffers. Does anybody have an idea how to solve it, or should I use labeled primers and Gene Scan to screen for this repeat (of course the products need to be separated anyway)


Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.