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production of monoclonal antibodies


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2 replies to this topic

#1 leelaram

leelaram

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Posted 05 December 2004 - 02:10 AM

hai pals,
can someone help me out by giving me any successful protocol for the
production of monoclonal antibodies. i have tries few protocols
but the hybridomas were not surviving
awaiting your suggestions
M. Naga Leelaram,
PhD student,
Indian Institute of Science,
Bangalore
India

#2 kiat2

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Posted 06 December 2004 - 02:14 AM

I tried many protocols too, I also tried using macrophage layer. But all my hybridoma did not survive (they did not multiply). You need the growth factor. I used the Hybridoma Fusion and Cloning Supplement (Catalogue no. 1363735) from Roche Diagnostic, and it worked. Good Luck.

#3 leelaram

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Posted 12 December 2004 - 04:57 AM

hai kait,
thanks for the suggestion
i will try it out and let you know the situation
thanks again
M. Naga Leelaram,
PhD student,
Indian Institute of Science,
Bangalore
India




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