I am using BamH1 and Nde1 to double digest the PCR product and Vector.The BamH1 and Nde1 are from NEB. I try using NEBuffer2 as buffer to digest,but the cleavege is poor.
Anyone has ever used these two REs together for double digestion? Which buffer do you use? Any tricks to increase the the efficency?
Edited by netnus, 02 December 2004 - 08:12 AM.