I need some urgent help here guys....
I did mini-prep of my plasmid clones using alakali-lysis method...yield close to 400- 700 ng/ul... good bands on the gel
Now to identify poitive clones I did restriction digestion with pvuII for one set of clones and HindIII for the next set. As control, I also digested a vector.
and I ran the digested plasmid side-by-side to the undigested ones....
and the digested ones have just disappeared: all i see is a smear-type band at the bottom of the gel. The undigested ones are fine and the control was also cut.
Is it that I have some nucleases in my miniprep or what?
Has anyone else faced this problem...I'd appreciate some help here
After restriction digestion my bands just disappear
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