I'm running SDS gels, I want to make up a 4x sample buffer. Do I just make up the 2x recipe in half the final volume?
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Posted 01 March 2002 - 03:53 AM
Posted 01 March 2002 - 09:32 AM
4x Sample buffer for (non-reduced gel): (for 10 mls use)
12% SDS 1.2g
40% Glycerol 4.0 ml
0.2 M tris-HCl pH7 2.0 ml of 1 M Stock
0.004% Bromophenol Blue 0.4 mg
4x Sample Buffer (for reduced gel):
Same volumes and amounts as #1 above but add to
solution 0.5 ml 2-Mercaptoethanol.