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What is the difference between 2x and 4x sample buffer?


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#1 AmyM

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Posted 01 March 2002 - 03:53 AM

Hi
I'm running SDS gels, I want to make up a 4x sample buffer.  Do I just make up the 2x recipe in half the final volume?

thanks


#2 jamescastle

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Posted 01 March 2002 - 09:32 AM

here's a recipe for 4x from gibco

4x Sample buffer for (non-reduced gel): (for 10 mls use)
      12% SDS              1.2g
      40% Glycerol        4.0 ml
      0.2 M tris-HCl pH7  2.0 ml of 1 M Stock
      0.004% Bromophenol Blue  0.4 mg

4x Sample Buffer (for reduced gel):
Same volumes and amounts as #1 above but add to
solution 0.5 ml 2-Mercaptoethanol.





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