Posted 01 December 2004 - 04:27 AM
Second, if a thermal cycler has not been used for several weeks, it seems that it works not so good. Has someone already observed this?
Posted 01 December 2004 - 12:34 PM
Some cyclers such as the Eppendorf Mastercycler have an option for altering the ramping speed in degrees per second, which can be useful for large templates and delicate reactions.
I havn't noticed this at all, but then the machines I have been using are all used at least once a week.
Posted 01 December 2004 - 08:05 PM
even i have observed the same, i followed the thermal programme of perkin elmer (which has been published) i didn't get the amplification in eppendorf. finally i have standardize the thermal program (annealing temp.)got the the amplification. which i have checked by restriction analysis for the conformation.