2 replies to this topic

[Deepak Joshi]

I exchanged the medium for A-549 cells 3 days before. At that time the cells were at phase of growth slightly lesser to confluent growth. When i observed the cells after 3 days of incubation at 37 C , i found all the cells were floating. I had to considered them  as dead cells.
Do the cells die because of the overgrowth or was there any contamination? I am confused. Can anybody found where i am mistaken?

[Josh]

I think it must be contamination played tricks.

[Jacen]

I have seen cells that become overconfluent die and lift off like that.  Esp. aggressive cell lines like B16.