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the cancer cell growth


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2 replies to this topic

#1 Deepak Joshi

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Posted 01 December 2004 - 03:57 AM

I exchanged the medium for A-549 cells 3 days before. At that time the cells were at phase of growth slightly lesser to confluent growth. When i observed the cells after 3 days of incubation at 37 C , i found all the cells were floating. I had to considered them as dead cells.
Do the cells die because of the overgrowth or was there any contamination? I am confused. Can anybody found where i am mistaken?

#2 Josh

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Posted 01 December 2004 - 05:02 PM

I think it must be contamination played tricks.

#3 Jacen

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Posted 07 December 2004 - 08:34 AM

I have seen cells that become overconfluent die and lift off like that. Esp. aggressive cell lines like B16.




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