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crosslinking of DNA after modification?


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#1 labtechie

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Posted 30 November 2004 - 09:58 AM

I am still trying to optimize my bisulfite modification protocol. I ran it on some size marker DNA in order to compare to the original and quantify how much DNA is lost during the treatment. Not only do I lose a significant amount of the DNA (about 90% less intensity on the gel), there is a bright band "stuck" in the well. Is this really long pieces of DNA that have been crosslinked or ligated during treatment?

Thank you for any help you can give.

#2 fuechschen

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Posted 01 December 2004 - 07:32 AM

Hi,
did you already try to increase the concentration of the hydroquinone as suggested by Raizis et al (Analytical Biochemistry 226, 161-166, 1995).

Good luck!




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