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mass balance in PCR


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#1 andreea

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Posted 30 November 2004 - 09:55 AM

Hello,

  how can I do a mass (molar) balance for the quantities entered and obtained through the polymerisation cycles ?
There must be a factor to correlate the theoretical results with the practical (the real quantity is smaller), a factor that includes all the influences on the reaction outcome(f).
  
  enter N= Conc.* volume entered ; (there are 30 cycles)
  exit: N*2'30*f  

  Any answer or suggestion is very much welcome :unsure:

  Andreea

#2 nabla

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Posted 30 November 2004 - 10:52 PM

exit: N*2'30*f -N-N*30 is the correct bilance. For estimation I woluld reduce the cycle number about 2-5 cycles. e.g. 27 instead of 30.

Edited by nabla, 30 November 2004 - 10:52 PM.


#3 andreea

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Posted 02 December 2004 - 10:24 AM

what is the value of [f] ?

Thanks

#4 nabla

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Posted 02 December 2004 - 10:51 PM

Sorry, no f : N*2'30 -N-N*30 !!  :huh:




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