mass balance in PCR
Posted 30 November 2004 - 09:55 AM
how can I do a mass (molar) balance for the quantities entered and obtained through the polymerisation cycles ?
There must be a factor to correlate the theoretical results with the practical (the real quantity is smaller), a factor that includes all the influences on the reaction outcome(f).
enter N= Conc.* volume entered ; (there are 30 cycles)
Any answer or suggestion is very much welcome
Posted 30 November 2004 - 10:52 PM
Edited by nabla, 30 November 2004 - 10:52 PM.