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Anybody has ever transfected EL4 cells with siRNA? - siRNA, microRNA and RNAi - BioForum

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Anybody has ever transfected EL4 cells with siRNA?


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#1 lsr

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Posted 30 November 2004 - 06:00 AM

:unsure: Hi!
are there any body have ever transfected EL4 cells with siRNA?or even any form of nucleic acid?
I have transfected for many of times with plasmid DNA such as eGFP and PLRTK,the efficiency is very poor. I tried Lipofectine2000 and electroporation, the later method seem to improve transfection of plasmid DNA,while it is a consuming method,and furthermore,I am afraid it would not work when I transfect EL4 with siRNA. I wonder if any one can give me some advice?Thank you!

#2 expresson_help

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Posted 18 January 2005 - 05:06 AM

NeoPhectin available from us works well in difficult to transform cell types such as endothelial cells and primary cells - more information at http://www.expresson...neophectin.html

David
ExpressOn

Edited by expresson_help, 18 January 2005 - 05:08 AM.





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