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[lsr]

Hi!
  are there any body  have ever transfected EL4 cells with siRNA?or even any form of nucleic acid?
  I have transfected for many of times with plasmid DNA such as eGFP and PLRTK,the efficiency is very poor. I tried Lipofectine2000 and electroporation, the later method seem to improve transfection of plasmid DNA,while it is a consuming method,and furthermore,I am afraid it would not work when I transfect EL4 with siRNA. I wonder if any one can give me some advice?Thank you!

[expresson_help]

NeoPhectin available from us works well in difficult to transform cell types such as endothelial cells and primary cells - more information at http://www.expresson...neophectin.html

David
ExpressOn

Edited by expresson_help, 18 January 2005 - 05:08 AM.