PCR blood old samples
Posted 28 November 2004 - 10:46 AM
Please, I have a problem and I need help! I have several blood samples obtained since 2002. These samples were mantained at 4ēC for several months, and at -20 ēC since may 2004. I´beem trying to extract DNA from these samples with DNAzol and the amount obtained was about 0.5 microgram/microliter from 100 microliter of blood. the samples were collected in EDTA tubes.
My question is: it is possible to perform PCR with these samples? The time of storage could degrade the DNA? the quantification in spetrophotometer could show contamination with proteins or other compounds masquerading the amount of DNA quantificated?
Thanks a lot for your attention!
Posted 28 November 2004 - 11:00 PM
Edited by nabla, 28 November 2004 - 11:00 PM.
Posted 01 December 2004 - 08:32 AM