Posted 27 November 2004 - 04:58 PM
Posted 28 November 2004 - 03:17 AM
are you sure that your plasmid has got FseI site ?you have told that you are getting problems in restriction. are u getting some sort of band on the gel after digestion? if so try to screen for the purity of the DNA. let me know if u get any banding pattern,
Posted 28 November 2004 - 04:29 AM
Check you have dissolved the DNA in water instead of TE buffer where EDTA interfere with restriction digestion.
Also prolong the incubation time
Hope you are taking sufficient enzyme for the digestion, pls check it alos