Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

restriction digestion


  • Please log in to reply
2 replies to this topic

#1 biochem_student

biochem_student

    member

  • Members
  • Pip
  • 5 posts
0
Neutral

Posted 27 November 2004 - 04:58 PM

i'm not sure if any of you are familiar with restriction enzyme Fse1. I have been having a lot of trouble with this enzyme because it doesn't always cleave my plasmid; it's also a really expensive enzyme. Does anyone have any background information on this RE? what is the optimal concentration of this enzyme i should use?

#2 sunil

sunil

    member

  • Members
  • Pip
  • 3 posts
0
Neutral

Posted 28 November 2004 - 03:17 AM

hi
are you sure that your plasmid has got FseI site ?you have told that you are getting problems in restriction. are u getting some sort of band on the gel after digestion? if so try to screen for the purity of the DNA. let me know if u get any banding pattern,

reg
sunil

#3 ravibiot

ravibiot

    member

  • Active Members
  • Pip
  • 24 posts
0
Neutral

Posted 28 November 2004 - 04:29 AM

HI
Check you have dissolved the DNA in water instead of TE buffer where EDTA interfere with restriction digestion.
Also prolong the incubation time

Hope you are taking sufficient enzyme for the digestion, pls check it alos

Best regards
RAVI




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.