2 replies to this topic

[DaveC]

Hello,

I need to remove a high concentration of small single and double stranded oligos (20-40bp) from a preparation of chromatin.
The chromatin pieces are 5-50 Kb in length, and fixed in formaldehyde.

Is it possible to use rate zonal or ispycnic centrifugation to efficiently separate the oligos from the chromatin?

I don't know much about this kind of centrifugation, so any advice/protocols would be really appreciated.

Thanks!
Dave

[mikew]

You can purchase columns from Biorad etc. or prepare your own resin available from biorad or other companies that exclude small pieces of DNA.

[netnus]

I always use Microcon Millipore centrifuge tubes. They always work well for me. In your case,you can choose the cutoff length <1Kbp.