Posted 25 November 2004 - 06:53 AM
I need to remove a high concentration of small single and double stranded oligos (20-40bp) from a preparation of chromatin.
The chromatin pieces are 5-50 Kb in length, and fixed in formaldehyde.
Is it possible to use rate zonal or ispycnic centrifugation to efficiently separate the oligos from the chromatin?
I don't know much about this kind of centrifugation, so any advice/protocols would be really appreciated.
Posted 06 December 2004 - 09:44 PM
Posted 07 December 2004 - 08:03 AM