Posted 24 November 2004 - 05:16 AM
There is an in-frame start codon upstream of the "official" start codon in my target gene. I have cloned a fragment that includes both - will translation be initiated at the first start codon after the promoter, or is there some form of cryptic signal to initiate translation only at "correct" start codons?
Posted 24 November 2004 - 09:04 AM
I think that there is a certain initiation signal upstream of the start codon such that the ribosome will bind there.
Once ribosome bind to the initiation signal, the ribosome will scan through the start codon and start the translation.
Therefore, I guess that it depends on where your initiation signal located.
Dunno this helps or not.
Posted 24 November 2004 - 09:07 AM
I think the expression vector will contain a ribosome binding site - I'll have to check that out.
Posted 25 November 2004 - 09:34 AM