Storage of 30% bis/acrylamide and SDS-PAGE gels
Posted 23 November 2004 - 11:30 PM
How long can one store 30% bis/acrylamide mixture in the fridge? And do I need to store it in the dark?
Also, how long can I keep SDS-PAGE gels in the fridge before I load and run them?
Posted 24 November 2004 - 05:47 AM
For a gel, I'd suggest not to prepare it more than one day prior to running samples. The best is to prepare it fresh. Still, if you want to cast it the day before, you put it in the "running box", with buffer, and soaked paper over it, every thing wraped in saranwrap, so every thing stay moist...
Hope I could help!!
Posted 24 November 2004 - 04:51 PM
Edited by SilverSta, 24 November 2004 - 04:53 PM.
Posted 25 November 2004 - 09:47 AM
You can easily cast your resolving gels and keep them in the fridge, as long as you keep the gels moist so they don't evaporate as suggested, and it’s not a problem to keep them for a week or so.
Posted 26 November 2004 - 06:31 AM
I do store acrylamide 30% at the fridge in a bottle protected from light (brown biorad bottles) and I have stored acrylamide several months like that!
Concerning acrylamide gels, I prepare them (12% gels for separating gel and 4% for stacking gel) the day before and store them after polymerization, at 4°C, with a parafilm on the top of the assembly system without removing combs.