Is mRNA spliced with very small fragment treated with siRNA?
Posted 23 November 2004 - 04:36 PM
Posted 23 November 2004 - 08:23 PM
Posted 25 February 2005 - 04:36 PM
Posted 26 February 2005 - 06:59 AM
According to the litterature, siRNA recognize a sequence of target mRNA (triple helix structure? remains unclear). The risc complex gets an endonuclease activity, then as akim said cuts tha target about 10 bp downstream of the recognition sequence. Then the two parts of cleaved mRNA are degraded by the cell machinery. And it's admitted that there is no transcription of the mRNA fragment.