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precipitation with ammonium sulfate


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#1 sandrine cottin

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Posted 23 November 2004 - 07:55 AM

I try to extract proteins from soil. I know I can use ammonium sulfate to precipitate proteins. But if I use ammonium sulfate on a solution containing proteins, humic acids, polysaccharides, and many components... what will I precipitate? Is it possible that ammonium sulfate precipitate humic acids ?

Can you help me?
:)
Sandrine
France

#2 win804

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Posted 23 November 2004 - 06:01 PM

In my opinion, ammonium sulfate precipitation technique will precipitate humic acids as well. This is similar to the salting out procedure.

As to how you are going to separate them, I am not sure. How about using the chromatography method?

Another point to consider is that: humic acids are insoluble in acidic condition (pH < 2) and soluble in the basic condition. Maybe you can look at this properties as well and try the multiple precipitation technique.

regards,
win804




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