Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Protein extraction from bacterial cells


  • Please log in to reply
1 reply to this topic

#1 telma

telma

    member

  • Members
  • Pip
  • 3 posts
0
Neutral

Posted 03 July 2002 - 04:13 AM

I urgently need a protocol that works well for protein extraction from gram-positive bacteria.  I am particularly looking to extract membrane and cytoplasmic fractions, which I intend to separate on  SDS-PAGE. Any suggestion on protocol information will be most appreciated.

(Edited by telma at 12:06 am on July 3, 2001)


#2 milanoj

milanoj

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 72 posts
0
Neutral

Posted 03 July 2002 - 06:52 AM

There are many permutations of protein extraction buffers and processes for any organism. A good start would be Protein Purification by Scopes.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.