3 replies to this topic

[puspa raj pandey]

i am a beginner in the field of cancer research, i am now culturing few cancer cells like aml-2 cells,hep g2 cells,t47d cells,a549 cells and 124 cells,,can anybody please send me the information on the culture of cancer cells????or u can also help me finding out the websites related to this field,,i have tried but i am not satisfied. thanking u in advance.


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[cellbiol]

Dear Puspa

All these cell lines can b routinely maintained in DMEM supplemented with 10% FBS or FCS. After the cells have become confluent u can subculture them in 1:3 to !:5 by tripsinization .

U can have more info on these cell lines at the official websites for atcc and ecacc. U can also read the bible of cell culture "Culture od Animal Cells" by R. Ian Freshney.

Good Luck

[antib]

see this web                                        http://www.dxy.cn/bb...=66&sty=1&age=0

[Jacen]

antib, on Dec 2 2004, 10:22 PM, said:

see this web                                        http://www.dxy.cn/bb...=66&sty=1&age=0

I tried that link but it doesn't work...