stem cell isolation
Posted 19 November 2004 - 11:39 AM
Posted 22 November 2004 - 04:07 PM
Posted 23 November 2004 - 08:35 AM
Posted 23 November 2004 - 02:50 PM
You raise an interesting idea that also has been proposed by many cancer biologists - they propose that the origin of cancer is from stem cells (called cancer stem cells) undergo indefinite self-renewal but lacking final differentiation (through certain mutatuion). There are many observations that support this idea. One of those is that many proto-oncogene, tumor suppressor, cell cycle regulators are all found actively expressed in stem cells and that "differentiation therapy" being a promising strategy.
However, one way to verify the tumor cells you isolated are really stem cell originated: differentiate them to see if they can be induced to do so (tumor regression as functional readout).
Remember that two typic characteristics of stem cells: self-renewal and differentiation potential.