Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo

E. coli culture contamination


  • Please log in to reply
2 replies to this topic

#1 rijnst

rijnst

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 19 November 2004 - 07:43 AM

hi guys,
i am just wondering is souce of the contamination in my E. Coli culture. the big yellow cell clusters are around. those clusters could be distroied by votex and then the whole liquid turn to yellow. the miniprep give a abnormally big pellet but there are only very small amount DNA inside. OD 280 is also low and the ratio 260/280 is normal.

#2 DevGrp

DevGrp

    member

  • Active Members
  • Pip
  • 19 posts
0
Neutral

Posted 19 November 2004 - 09:26 AM

Sounds like Staphylococcus aureus to me. I'd streak your E.coli out, get a single white colony and start again. You may have preped a mixed E.coli / staph culture which would explain your low plasmid yeild.

#3 madhubiotech

madhubiotech

    member

  • Active Members
  • Pip
  • 12 posts
0
Neutral

Posted 20 November 2004 - 09:29 PM

hi

yellow clusters might even be micrococcus contamination. it ur e.coli is recA u can screen by mutagenesis with uv and purify the colonies.

madhan




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.