Salmon sperm DNA
Posted 18 November 2004 - 03:46 PM
I posted the following question elswhere but i hadn't no response yet ! so, i post it here hoping obtain your opinions !!
I'd like to know if Salmon Sperm DNA is really necessary in the DNA/RNA prehybridization procedures? what happens if i don't use it? and when it's used, does it affect the hybridization efficiency while excercing a competitive role with the specific sequence and diminish the good match probe/target? Does it come down to cover the DNA and make it hardly accessible to be hybridized with it's complementary strand?
Posted 19 November 2004 - 09:05 AM
I have found using Denharts or Church pre-hybridisation buffer without salmon (or herring) sperm DNA works just as well.
Posted 19 November 2004 - 11:18 AM
Posted 24 December 2004 - 02:15 AM
beacause in northern blot what you will detect is mRNA which has 18 s 28s rRNA . the rRNA can hybride with any probe for its homolous.
Posted 28 December 2004 - 09:28 PM
Posted 29 December 2004 - 11:49 AM