How many cell passages to be safe ?
Posted 18 November 2004 - 10:16 AM
I don't have any experience in cell culture at all and I've just started to culture and transfect some cells for a few weeks. People from my lab gave me some SK-N-SH and C6 glioma cells.
The thing is nobody told me how many passages these cells have already been through... and this doesn't seem to worry anyone in the lab.
The cells look OK but I'm just wondering if they might lose some of their properties bit by bit with many passages...
What do you think ? Does anyone have experience with these cell lines especially ?
Thanks for your answers.
Posted 19 November 2004 - 05:55 AM
Hope this helped...
Posted 19 November 2004 - 07:39 AM
Posted 19 November 2004 - 08:15 AM
After this we always went back to the early passage stocks every 15 passages.
I think different cells behave in different ways. always keep an eye out for changes in the phenotype you are studying.
Posted 19 November 2004 - 09:17 AM
Posted 06 December 2004 - 07:39 AM
it is hard to say whether it is safe or not , because different cells have different situation cultured in vitro
Posted 07 December 2004 - 03:32 PM