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[Huanghui Tang]

Hi, pcrman; why the forward primer is designed in such a way that it won't be complement to either strand (sense or antisense) after bisulphite conversion? e.g
(example from Nucleic acids research 1994,22:2995)

original strand: 5' ----TCTA GAG TCCC (Antisense:3'---AGAT CTC AGGG 5')
primer             : 5' ----TTTA GAG  T TTT

Thanks!  

[pcrman]

Before PCR,  the DNA has to be modified using sodium bisulfite to convert unmethylated C to U.

original strand:   5' ----TCTA GAG TCCC [C]GT
modified strand  5'---- TUTA GAG TUUU  CGT
primer :              5' ----TTTA GAG T TTT