Posted 18 November 2004 - 04:23 AM
How Paraformaldehyde (PFA 0.5%) "fixes"the cell before a flow cytometric analysis??????
Posted 19 November 2004 - 07:36 AM
Posted 29 November 2004 - 06:32 AM
Regarding cell fixation, I've been having problems trying to detect beta1 integrin in an epithelial cell-line by immunofluorescence. In your experience, does fixation with 0.2% paraformaldehyde for 10 mins at RT alter antigenic epitopes, especially for membrane proteins?
Thanks for your insight.
Posted 07 December 2004 - 08:49 AM
Posted 07 December 2004 - 02:57 PM
Thanks all of you for your interest in my question.
I stain cells with monoclonal antibody cell surface markers and analyze them by using flow cytometry. Before any procedure, the protocol specifies that we should use PFA 0.5% to "fix" the cell.
My specific question was how does PFA fix the cell? Is the protein crosslinking still a possible answer for this?