I'm working on a degenerate PCR for the detection and typing of enteroviruses. My problem is that my negative controls shows a band of about 200 bp (my target is 340 bp) that is very fague and an even faguer 340 bp band. First I thought this was contamination, but than I tested the same RT -samples with specific primers and all my negatives stayed negative. What is up with my negatives in the degenerate PCR?
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