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making chemically competent BL21 pLysS cells

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#1 Cindy



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Posted 17 November 2004 - 09:53 AM

I made up a batch of what I thought were chemically competent BL21 pLysS cells using 50 mM calcium chloride solution, but I tried transforming them and ended up getting 2 colonies on my positive control DNA plate and none on the plate that I wanted to actually transform. Is there a good protocol for making these cells competent, or should I stick to the calcium chloride protocol that I already have? Any help would be greatly appreciated :)

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