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Rhamnolipid production test


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#1 ravibiot

ravibiot

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Posted 17 November 2004 - 09:44 AM

hello,

i work on microbial degradation of hydrocarbons, its relevent to screen the degradative baceria for the production of rhamnolipids which reduces the surface tension in liquid culture medium.
i have a problem in the palte assay, wehre getting a blue colour zone formation around the colonies on SW blue agar (seigmund and wagner, 1991) which indicates positive reactrion. surprize is that, i did not get any blue zone for control strain also (Pseudomonas CHAO, CHAO660) which are reported for the same.

if some one can get me this article i am greatful for their help.
Siegmund, I., and Wagner, F. (1991) Biotechnology Techniques 5, 265-268

so, plese some one related to this field are highly appreciated for their help.

thank you in advance

sincerely,
ravi,
INDIA

Edited by ravibiot, 28 November 2004 - 03:45 AM.





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