Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Restriction enzymes cut reaction


  • Please log in to reply
1 reply to this topic

#1 hongthanh1112

hongthanh1112

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 16 November 2004 - 07:47 PM

[FONT=Arial][SIZE=1]
Hi! I'm wonderful If anyone has answer as to "Why I don't cut plasmid pET22b(+) by BamHI and XhoI. When I cut it with normal protocol then I don't ligate with 500 bp fragment"

#2 Janina

Janina

    member

  • Active Members
  • Pip
  • 25 posts
0
Neutral

Posted 17 November 2004 - 02:05 AM

I have also problems with the pET22b vector... We found out that it is possible to cut by sequentiell digestion but not by double digest. Perhaps this will help you...




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.