Can shRNA be transfected without vector...??
Posted 16 November 2004 - 07:45 PM
I'm new to this and am ultimately wanting to have an inducible vector in transgenic mice - to switch gene on and off during development (not sure if this is aiming too high?!). Initially, to check for knockdown I was going to just transfect some siRNAs into cells - is this a good first step, or should I make the shRNA and clone into vector and test function at this point? Is there a way to transfect the hairpin into the cells without vector perhaps...??
Am grateful for any comments, sorry to be so wordy...
Posted 16 November 2004 - 10:37 PM
Once you find a reliable target with synthesized siRNA, then you can construction your vector to achieve long-term silencing.
Transfecting hsRNA longer than 30 bp may cause interferon response. So that is not a choice.
Edited by pcrman, 16 November 2004 - 10:40 PM.
Posted 19 November 2004 - 11:27 PM
so catjo, u r working with shRNA as well ? may i know where are u from?
Posted 23 November 2004 - 06:22 PM
Posted 01 June 2009 - 11:27 AM