Freezing down cell lysate
Posted 16 November 2004 - 09:43 AM
Does anyone know whether its possible to freeze down whole cell lysates (Produced using the Nonidet P-40 detergent lysis buffer) for a time?
I am going on holiday for a long weekend and have transfected cells which will be ready on the Friday. I was planning on freezing the lysate and immunoprecipitating stuff on the Tuesday.
Has anyone tried this before and if so did it work?
Posted 17 November 2004 - 06:44 AM
Other than that, I wouldn't hesitate much freezing my lysate o/we and IP the week after... not much moves at -20°C!!
Posted 18 November 2004 - 01:06 AM