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Theory question on protein phosphorylation


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2 replies to this topic

#1 louisj23

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Posted 16 November 2004 - 05:26 AM

This is not an experimental question but a theoretical one:
I have asked this in another post but it seems to be misunderstood.
Q: Is it possible to have a protein phosphorylated at sites that do not affect its mobility as visualized by SDS-PAGE?
The reason for asking this is that it is the basis for a specific aim in a fictional grant proposal that I am presenting to my graduate comittee.
Thanks

#2 Sprag

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Posted 16 November 2004 - 08:31 AM

I wouldn't think so, but the changes might be so minor that it can be hard to detect the differences... But no, phosphorylation should change both molecular weight (Western) and the pH (IEF)..

#3 Simonsays

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Posted 17 November 2004 - 06:45 AM

check your other post

Simon




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