PCR of unknown DNA
Posted 15 November 2004 - 08:14 AM
Posted 24 November 2004 - 02:02 AM
you can order oligonucleotides and use them as linkers
Important: do you use a digest which you want to amplify? Then use compatible linkers. Note that oligonucleotides normally are not 5'phosphorylated, this is in fact an advantage.
If you want to ligate linkers to blunt-end DNA, make sure you have a double-stranded linker.
Posted 25 November 2004 - 08:29 AM
Vectorette primers seem like such a clever idea, yet I can find so few papers where they are used. Is there a reason why it hasn't taken off?
and there are improvements called vectorette, sprinklerette etc.