Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Quantification of PCR using delta-delta Ct method.


  • Please log in to reply
1 reply to this topic

#1 neven

neven

    member

  • Active Members
  • Pip
  • 7 posts
0
Neutral

Posted 15 November 2004 - 01:50 AM

One of the most used quantification methods in PCR is the "delta-delta Ct" method. In this method we use the Ct-value given from the fixed threshold value and detected product of the PCR-reaction.

To me it seems that the "Ct-value" is a exponetial value. Is this right? Does anyone have any comment on this?

#2 Charon

Charon

    member

  • Active Members
  • Pip
  • 25 posts
0
Neutral

Posted 16 November 2004 - 04:31 AM

Of course the values are exponential as the PCR reactions are exponential functions..
In the delta-delta ct method usually the expression is normalized against a housekeeping gene, the given difference (delta-ct) is then substracted from the calibrator sample resulting in the delta-delta-ct value.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.