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why is LDH secreted


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#1 carl_ulrich

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Posted 11 November 2004 - 04:57 AM

Can anyone explain to me why LDH is secreted from eukaryotic cells during oxidative stress?

#2 DevGrp

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Posted 12 November 2004 - 03:01 AM

LDH is released from cells with damaged / destroyed cell membranes during cell death. An LDH kit made by Roche is a standard way to look at cell membrane damage / cell necrosis

Hydrogen peroxide (in the cells I have worked on) will at low concentrations cause apopotosis while at higher concentrations it causes necrosis.
For a nice graph showing this see figure 1 J Endocrinology. 1999 May;161(2):199-210. Functional inactivation of the oestrogen receptor by the antioestrogen, ZM 182780, sensitises tumour cells to reactive oxygen species. Newton CJ, Drummond N, Burgoyne CH, Speirs V, Stalla GK, Atkin SL.

I can email you the PDF if you can't access it.

I expect you are inducing oxidative stress which is causing cell death. Have a look at your cells under the microscope you may be able to cell apoptosis / necrosis.

I was interested that you had also detected an increase in LDH transcription after H2O2 treatment. This I never measured and would have to think about.

Hope this helps

Ian




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