1 reply to this topic

[carl_ulrich]

I would like to know if addition of hydrogen peroxide to a growth medium reduces the oxygen content in the medium, leading to hypoxia or any sort of oxygen deficiency.  Ldh (lactate dehydrogenase) transcription is induced during oxidative stress when I add H2O2 to my cell culture.  From the literature I read, however, that LDH expression is induced during oxygen deficiency, which makes more sense to me.  Can anyone explain to me why LDH expression is induced during oxidative stress?  Thanks

[madhubiotech]

hi
as far it goes to my knowledge, during oxidatinve stress u get lactate getting accumulated as a result of anaerobic process and this could certainly induce LDH genes . i may be right. i dont know exactly. is that answer convincing for u. refer Biochemistry by Lehninger or Stryer for further details
regards
madhan shankar
Biotechnology Head
DR NGP Arts & Science College
coimbatore