Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

O2 vs H2O2


  • Please log in to reply
1 reply to this topic

#1 carl_ulrich

carl_ulrich

    member

  • Members
  • Pip
  • 4 posts
0
Neutral

Posted 11 November 2004 - 04:54 AM

I would like to know if addition of hydrogen peroxide to a growth medium reduces the oxygen content in the medium, leading to hypoxia or any sort of oxygen deficiency. Ldh (lactate dehydrogenase) transcription is induced during oxidative stress when I add H2O2 to my cell culture. From the literature I read, however, that LDH expression is induced during oxygen deficiency, which makes more sense to me. Can anyone explain to me why LDH expression is induced during oxidative stress? Thanks

#2 madhubiotech

madhubiotech

    member

  • Active Members
  • Pip
  • 12 posts
0
Neutral

Posted 11 November 2004 - 07:03 AM

hi
as far it goes to my knowledge, during oxidatinve stress u get lactate getting accumulated as a result of anaerobic process and this could certainly induce LDH genes . i may be right. i dont know exactly. is that answer convincing for u. refer Biochemistry by Lehninger or Stryer for further details
regards
madhan shankar
Biotechnology Head
DR NGP Arts & Science College
coimbatore




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.