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electroporation question


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#1 GT(N)T

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Posted 08 November 2004 - 10:41 AM

My lab is considering employing Travis Glenn's protocols for microsatellite isolations. One step requires electroporation in the transformation of E coli. A couple of questions:

1) what electroporation apparatus is available to a budget-conscious lab?

2) are there alternatives to this step in the protocol?

Thanks for any suggestions!

#2 Charon

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Posted 09 November 2004 - 02:58 AM

Why, sure. CaCl2-heat shock transformation should work, too. And it costs next to nothing. I only browsed over the protocols but it didn't occur to me that they were too large to get them without electroporation.




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