Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo

Isolation of small RNAs


  • Please log in to reply
1 reply to this topic

#1 kueffer

kueffer

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 08 November 2004 - 08:17 AM

Hi

I'm doing some research for small RNA isolation (specificly miRNAs). The Kits from Ambion don't describe the special properties of specific small RNA isolation.
Does anybody know a description or explanaition why for example EtOH is used instead of Isopropanol? And three volumes of EtOH is used instead of two?
Why Na-acetat is used and not anything else?

Stefan

#2 pcrman

pcrman

    Epigenetist

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,160 posts
65
Excellent

Posted 08 November 2004 - 10:05 PM

Hi Stefan,

I think those are just basic principles for nucleic acid precipitation and nothing is very special for small RNAs. For example, in Stratagen's microRNA isolation kit instruction, isopropanol is used instead of ethanol.

In addition, this thread may be useful:

http://www.protocol-...?showtopic=3874




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.