Negative control for MSP
Posted 07 November 2004 - 11:18 PM
Posted 08 November 2004 - 02:58 PM
There are two possibilities:
2) nonspecific pairing. How many non-CpG Cs are in the M primer? Try raising the annealing temperature a bit.
Posted 08 November 2004 - 06:13 PM
Posted 09 November 2004 - 02:25 PM
do you think my ladder caused by amplicon prime itself in some way to generate concatanated products which will accumulate in number and size as the PCR cycles and form ladders? I checked my primer, and found there are several internal repeats, which would allow the PCR product to act as a primer on itself.