I am trying to run a PCR on gill samples since 6 months. I finally got it to wotk after changing annealing temperature, Taq and dNTPs.
Now I have 9 god-looking genomic DNA samples, which all have around the same concentrations and look nice on the gel, but only one works!!!!!!!
What am I doing wrong? I retried using the same samples again with different amounts of DNA, but nothing works! It just does not seem normal!
If anybody could help, that would be awsome!!!!!
Edited by bioforum, 07 November 2004 - 03:14 PM.