the DNA is not symmetrical
Posted 06 November 2004 - 08:49 AM
Do you agree with me?
Posted 06 November 2004 - 09:13 AM
DNA methylation is supposed to be symmetric on both strands. One possiblility that counts for the discrepancy is that your MSP primers are not specific enough to anneal only to methylated CpG sites, which is not uncommon especially if your PCR conditions are not optimized.
Edited by pcrman, 06 November 2004 - 09:15 AM.
Posted 08 December 2004 - 02:57 PM