why don't do MSP product sequencing
Posted 05 November 2004 - 02:46 PM
Posted 05 November 2004 - 03:25 PM
I figure that people who sequence their MSP products wants to confirm if their MSP results are true or not. Actually, they cannot. No matter you sequence MSP or USP products, they are amplified with bias and the methylation status of CpG sites within the PCR product will never tell you the true picture. If you sequence MSP products, you will get much higher methylation level, USP lower methylation.
Posted 06 November 2004 - 08:19 AM
do you agree with me?