Jump to content

  • Log in with Facebook Log in with Twitter Log In with Google      Sign In   
  • Create Account

- - - - -

Control for primer extension

Started by Praveen Bawankar, Nov 04 2004 08:52 PM

  • Please log in to reply
2 replies to this topic

#1 Praveen Bawankar

Praveen Bawankar

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 04 November 2004 - 08:52 PM

Hi
Any one plz tell me what control should i take for primer extension of RNA.

#2 paulina

paulina

    member

  • Active Members
  • Pip
  • 17 posts
0
Neutral

Posted 13 November 2004 - 09:07 AM

For negative control, use no RNA or RNA from cells that don't express your gene.

For positive control,  maybe include some gene for which transcription start site is known.

#3 Mone13

Mone13

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 22 December 2004 - 06:33 AM

I have looked for a positive control, but I canīt find a gene which transcription start point is known.
Back to Molecular Biology · Next Unread Topic →


  1. BioForum
  2. → Protocols and Techniques Forums
  3. → Molecular Biology
  4. Privacy Policy
Home - About - Terms of Service - Privacy - Contact Us

©1999-2012 Protocol Online, All rights reserved.