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How to find the transcription start site of an unknown gene?


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#1 sallymm

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Posted 03 November 2004 - 03:02 PM

How to find the transcription start site of an unknown gene?

how many method??

Please email to :

sallymmxhl@yahoo.com

Thanks everyone!

#2 smesme

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Posted 04 November 2004 - 06:33 AM

Hi Sally,

One quick method is to search and align all EST's you can find for the gene in genbank. If you find 2 or 3 in the most 5' end that initiate at the same base, it is probably the startsite.

regards,

Søren

Søren M. Echwald, MSc., Ph.D.
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Exiqon A/S
Bygstubben 9
DK-2950 Vedbaek
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www.ProbeLibrary.com
One Real-time PCR kit, which covers 38.565 genes




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