2 replies to this topic

[pisa1234]

is it ok for the following methprimer
AAATGGAGAACCGCGCTGGCGTGGAGTTGATACAGAAACACTGCAAGGGCTGGGGAC
AGGGCTTAGTTCGTGGAATACACAGGCAGCACCACCAACCCGGCTCTCCGGAAAAGA
GAAAGTCCAGTGAGAAAGGTGAAAACTGACAAGGCACCGTTCTCCGACTTGCTGAAA
AAAAGACAATGCAAATAGAGCAGGGTCCAAGGCGGAAGAAAATCAGAGAAAAGCAGG
CAGCCAAGCGTGGCGAACACAGCTCTCGGGGCCACCCCGGAGGGGACCCGCGGCG
GCCGCAGCGGGGCGGAGGCCGAAGCCGAGGCCGCCCCGGAACCAGAGAGCCAGG
CGGGAAGTGCACAAAGGCACGAGGCCACGCCCAGGAGGGGCGGGCCCTTTGGCCG
GAAGGGGGGGGGCCGGCGCGCGGGGCCGGTCGCCTAGGCAACGGGCTCGCGTGG
CGTCCAGGCTTCTCAGAAAGCCCAAAATCCGGGAGCTTTGGGAGGGTAGAGGGGCG
ACGCGGGGGAGGGCGGTCCGGGGAGCTGAATGGCCTCAGGACGCCGGCCGACCGG
GTGTCTGCATACTGTGGGCGGCCTTTCCAAGTGTGGGGAGCGG

BSP
Left  primer       206   30   56.49   43.33   5  GAAGAAAATTAGAGAAAAGTAGGTAGTTAA
   Right primer       473   26   53.69   50.00   8  TTTTAAACTTTCTAAAAAACCTAAAC
   Product size: 268, Tm: 72.0, CpGs in product: 29

MSP
1  Left  M primer     334   24   59.83   62.50   4  TAGGCGGGAAGTGTATAAAGGTAC
   Right M primer     469   25   59.58   64.00   8  AAACTTTCTAAAAAACCTAAACGCC
   Product size: 136, Tm: 70.6
   Left  U primer     334   26   59.30   61.54   4  TAGGTGGGAAGTGTATAAAGGTATGA
   Right U primer     470   26   55.95   61.54   8  TAAACTTTCTAAAAAACCTAAACACC
   Product size: 137, Tm: 68.7


AGACAATGCAAATAGAGCAGGGTCCAAGGCGGAAGAAAATCAGAGAAAAGCAGGCAG
CCAAGCGTGGCGAACACAGCTCTCGGGGCCACCCCGGAGGGGACCCGCGGCGGCC
GCAGCGGGGCGGAGGCCGAAGCCGAGGCCGCCCCGGAACCAGAGAGCCAGGCGG
GAAGTGCACAAAGGCACGAGGCCACGCCCAGGAGGGGCGGGCCCTTTGGCCGGAA
GGGGGGGGGCCGGCGCGCGGGGCCGGTCGCCTAGGCAACGGGCTCGCGTGGCGT
CCAGGCTTCTCAGAAAGCCCAAAATCCGGGAGCTTTGGGAGGGTAGAGGGGCGACG
CGGGGGAGGGCGGTCCGGGGAGCTGAATGGCCTCAAGCGG

BSP
   Primer            Start Size  Tm      GC%   'C's  Sequence
1  Left  primer        32   30   56.49   43.33   5  GAAGAAAATTAGAGAAAAGTAGGTAGTTAA
   Right primer       299   26   53.69   50.00   8  TTTTAAACTTTCTAAAAAACCTAAAC
   Product size: 268, Tm: 79.2, CpGs in product: 29
MSP
   Primer            Start Size  Tm      GC%   'C's  Sequence
1  Left  M primer     160   24   59.83   62.50   4  TAGGCGGGAAGTGTATAAAGGTAC
   Right M primer     295   25   59.58   64.00   8  AAACTTTCTAAAAAACCTAAACGCC
   Product size: 136, Tm: 75.1

[pcrman]

They seem OK to me. Just go ahead with your PCR.

[pisa1234]

Thank you.