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BSP and MSP primer check


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#1 pisa1234

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Neutral

Posted 03 November 2004 - 08:56 AM

is it ok for the following methprimer
AAATGGAGAACCGCGCTGGCGTGGAGTTGATACAGAAACACTGCAAGGGCTGGGGAC
AGGGCTTAGTTCGTGGAATACACAGGCAGCACCACCAACCCGGCTCTCCGGAAAAGA
GAAAGTCCAGTGAGAAAGGTGAAAACTGACAAGGCACCGTTCTCCGACTTGCTGAAA
AAAAGACAATGCAAATAGAGCAGGGTCCAAGGCGGAAGAAAATCAGAGAAAAGCAGG
CAGCCAAGCGTGGCGAACACAGCTCTCGGGGCCACCCCGGAGGGGACCCGCGGCG
GCCGCAGCGGGGCGGAGGCCGAAGCCGAGGCCGCCCCGGAACCAGAGAGCCAGG
CGGGAAGTGCACAAAGGCACGAGGCCACGCCCAGGAGGGGCGGGCCCTTTGGCCG
GAAGGGGGGGGGCCGGCGCGCGGGGCCGGTCGCCTAGGCAACGGGCTCGCGTGG
CGTCCAGGCTTCTCAGAAAGCCCAAAATCCGGGAGCTTTGGGAGGGTAGAGGGGCG
ACGCGGGGGAGGGCGGTCCGGGGAGCTGAATGGCCTCAGGACGCCGGCCGACCGG
GTGTCTGCATACTGTGGGCGGCCTTTCCAAGTGTGGGGAGCGG

BSP
Left primer 206 30 56.49 43.33 5 GAAGAAAATTAGAGAAAAGTAGGTAGTTAA
Right primer 473 26 53.69 50.00 8 TTTTAAACTTTCTAAAAAACCTAAAC
Product size: 268, Tm: 72.0, CpGs in product: 29

MSP
1 Left M primer 334 24 59.83 62.50 4 TAGGCGGGAAGTGTATAAAGGTAC
Right M primer 469 25 59.58 64.00 8 AAACTTTCTAAAAAACCTAAACGCC
Product size: 136, Tm: 70.6
Left U primer 334 26 59.30 61.54 4 TAGGTGGGAAGTGTATAAAGGTATGA
Right U primer 470 26 55.95 61.54 8 TAAACTTTCTAAAAAACCTAAACACC
Product size: 137, Tm: 68.7


AGACAATGCAAATAGAGCAGGGTCCAAGGCGGAAGAAAATCAGAGAAAAGCAGGCAG
CCAAGCGTGGCGAACACAGCTCTCGGGGCCACCCCGGAGGGGACCCGCGGCGGCC
GCAGCGGGGCGGAGGCCGAAGCCGAGGCCGCCCCGGAACCAGAGAGCCAGGCGG
GAAGTGCACAAAGGCACGAGGCCACGCCCAGGAGGGGCGGGCCCTTTGGCCGGAA
GGGGGGGGGCCGGCGCGCGGGGCCGGTCGCCTAGGCAACGGGCTCGCGTGGCGT
CCAGGCTTCTCAGAAAGCCCAAAATCCGGGAGCTTTGGGAGGGTAGAGGGGCGACG
CGGGGGAGGGCGGTCCGGGGAGCTGAATGGCCTCAAGCGG

BSP
Primer Start Size Tm GC% 'C's Sequence
1 Left primer 32 30 56.49 43.33 5 GAAGAAAATTAGAGAAAAGTAGGTAGTTAA
Right primer 299 26 53.69 50.00 8 TTTTAAACTTTCTAAAAAACCTAAAC
Product size: 268, Tm: 79.2, CpGs in product: 29
MSP
Primer Start Size Tm GC% 'C's Sequence
1 Left M primer 160 24 59.83 62.50 4 TAGGCGGGAAGTGTATAAAGGTAC
Right M primer 295 25 59.58 64.00 8 AAACTTTCTAAAAAACCTAAACGCC
Product size: 136, Tm: 75.1

#2 pcrman

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Excellent

Posted 03 November 2004 - 09:22 AM

They seem OK to me. Just go ahead with your PCR.

#3 pisa1234

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Neutral

Posted 03 November 2004 - 09:27 AM

Thank you.




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