growth media question
Posted 02 November 2004 - 11:13 AM
Posted 04 November 2004 - 04:10 AM
Basically you should check your manicacturer try to find out precisely what it is.
Possibly the ingredient you are using is a mix.
And yes changes in the medium can result in growth differences (though depending on your species/strain it is possible that they are not visible) and almost always leads to a change in the proteome. If you want to purify a specific protein it might not be important (especially for instance if working with an overexpression vector). However whole proteome approaches (2d gel for instance) are very sensitive to changes in medium composition.
Posted 05 November 2004 - 10:37 AM
Posted 08 November 2004 - 05:08 AM
Alternatively you could have galactose contamination (doubt it, though).