Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Rephosphorylation of SAP treated DNA fragment


  • Please log in to reply
1 reply to this topic

#1 petraparker

petraparker

    member

  • Members
  • Pip
  • 3 posts
0
Neutral

Posted 02 November 2004 - 12:58 AM

Hi!!!!
I SAP (shrimp alkaline phosphatase) treated a DNA fragment and want to redo (phosphorylise?!) this. I want to blunt-end clone this fragment in a SAP treated vector (vector cut and then Klenow-Pol filled up).
Can anybody help me!!??!!??
Thank in advance!!
PP

#2 jadefalcon

jadefalcon

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 223 posts
1
Neutral

Posted 02 November 2004 - 08:37 AM

Polynucleotide kinase (PNK) will add phosphate residues to dephosphorylated DNA ends. available from common suppliers like Roche or NEB.

mike
--- He who finds typos may keep them! ---




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.